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If you have any questions, please call: US residents 1-800-BRAUN-11. Repairs on electric appliances (including cord replacement) must only be carried out by author- ized service centers. Faulty, unqualified repair work may cause accidents or injury to the user. Limited Warranty Braun warrants this Braun appliance to be free of defects in material and workmanship for a period of one year from the date of original purchase at retail. Limited 1-year Warranty In the event a Braun appliance fails to function within the specified warranty period because of defects in material or workmanship, and the consumer returns the unit to an. Luego de la limpieza, vuelva a colocar la tapa. Sujeto a cambio sin previo aviso. Post your question here in this forum. We're committed to dealing with such abuse according to the laws in your country of residence. When you submit a report, we'll investigate it and take the appropriate action. We'll get back to you only if we require additional details or have more information to share. Note that email addresses and full names are not considered private information. Please mention this; Therefore, avoid filling in personal details. Please enter your email address. If you are here, it was rather the case. However, you are not the only person having problems with keeping the operation manual of all household devices. Below are few guidelines regarding how and why you should collect the product manuals. However, we believe that the manuals should include the most important and needed information about Braun 4161, not to discourage the user to read. Obviously, if a device Braun 4161 has multiple advanced functions, we will not avoid a high amount of information in this document. Then, it will be much easier to find it than look through the purchase boxes which have already been thrown away by you or any other of household members. It will be enough to clear the drawer once in a year and throw away any manuals of the devices you do not use anymore. http://finanteca.com/userfiles/briggs-and-stratton-intek-engine-repair-manual.xml

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Thus, you can avoid storing any unnecessary documents and keep only those which are valid. You can also download and print the manual Braun 4161 to place it in your drawer. Learn more - opens in a new window or tab This amount is subject to change until you make payment. For additional information, see the Global Shipping Programme terms and conditions - opens in a new window or tab This amount is subject to change until you make payment. If you reside in an EU member state besides UK, import VAT on this purchase is not recoverable. For additional information, see the Global Shipping Programme terms and conditions - opens in a new window or tab Learn More - opens in a new window or tab Learn More - opens in a new window or tab Learn More - opens in a new window or tab Learn More - opens in a new window or tab Learn More - opens in a new window or tab The item may have some signs of cosmetic wear, but is fully operational and functions as intended. This item may be a floor model or an item that has been returned to the seller after a period of use. See the seller’s listing for full details and description of any imperfections. You're covered by the eBay Money Back Guarantee if you receive an item that is not as described in the listing. Find out more about your rights as a buyer - opens in a new window or tab and exceptions - opens in a new window or tab. Contact the seller - opens in a new window or tab and request post to your location. Please enter a valid postcode. Please enter a number less than or equal to 1. We may receive commission if your application for credit is successful. Terms and conditions apply. Subject to credit approval. I only accept cheques and postal orders etc.I would prefer payment by Paypal as it is quicker, but I do except cheques and postal orders in UK currency. We may receive commission if your application for credit is successful. All Rights Reserved. User Agreement, Privacy, Cookies and AdChoice Norton Secured - powered by Verisign. http://drjihsderma.com/pics/briggs-and-stratton-intek-23-hp-manual.xml

Ask your question here. Provide a clear and comprehensive description of the issue and your question. The more detail you provide for your issue and question, the easier it will be for other Braun Citromatic MPZ 9 owners to properly answer your question. Ask a question About the Braun Citromatic MPZ 9 This manual comes under the category Juicers and has been rated by 1 people with an average of a 5.6. This manual is available in the following languages: English, Dutch, German, French, Spanish, Italian, Swedish, Portuguese, Danish, Polish, Norwegian, Finnish, Czech, Turkish, Slovak, Hungarian. Do you have a question about the Braun Citromatic MPZ 9 or do you need help. Ask your question here Braun Citromatic MPZ 9 specifications Brand ManualSearcher.com ensures that you will find the manual you are looking for in no time. Our database contains more than 1 million PDF manuals from more than 10,000 brands. Every day we add the latest manuals so that you will always find the product you are looking for. It's very simple: just type the brand name and the type of product in the search bar and you can instantly view the manual of your choice online for free. ManualSearcher. com If you continue to use this site we will assume that you are happy with it. Read more Ok. In our database you will get access to 2017 official Braun documents in which, you can find the necessary and reliable information right now. In addition, you can always contact other users with specific issue. Watch The Repair Video Grid is 1-inch square. Can't locate ours and would love to keep using the juicer. Thank you for your question and good luck with your repair! We hope this helps. Best Regards. Thank you, Sincerely, Judy The model number you provided is not recognized. Please verify the number you provided us. If it is correct, we recommend contacting the manufacturer and seeing if they could provide you with a part number, and then you are always welcome to check back to see if we carry the part. http://gbb.global/blog/02-router-manual

We hope this helps. Please contact us anytime. If not where I get a replacement cone? We do apologize but we were not able to locate a parts list for your model number, (MPZ2 No 4979-Braun Citromatic). For further assistance, we recommend calling the manufacturer of the equipment directly at 800-272-8611. They may be able to provide you with the part number that you need. Once you have this information, please feel free to check back with us for pricing and availability information. Best Regards. Just replaced the missing cone with the new one. Did you find this story helpful. Yes No Thanks for voting and helping fellow customers! 1 of 1 people found this instruction helpful Tool Type: Juicer Parts Used: Cone - BR64161627 Repair Difficulty: Easy Time Spent Repairing: Less than 15 minutes Tools Used:, Nothing. My old juicer cone had missing teeth Simply attached the cone to juicer. Love finally being able to use it again. Did you find this story helpful. Yes No Thanks for voting and helping fellow customers. Tool Type: Juicer Parts Used: Cone - BR64161627 Repair Difficulty: Easy Time Spent Repairing: Less than 15 minutes Tools Used: Needed two parts for Braun Juicer.ol Just inserted the parts into proper receptacle. Did you find this story helpful. Yes No Thanks for voting and helping fellow customers. Tool Type: Juicer Parts Used: Cone - BR64161627 Bowl - Mpz 6 (350Ml) - BR64161622 Repair Difficulty: Easy Time Spent Repairing: Less than 15 minutes Tools Used:, None Missing parts Reassembled Did you find this story helpful. Yes No Thanks for voting and helping fellow customers! Show All Reviews. Please try again.Please try again.In order to navigate out of this carousel please use your heading shortcut key to navigate to the next or previous heading. Please adjust the filters and try again. Try adjusting your filters.In order to navigate out of this carousel please use your heading shortcut key to navigate to the next or previous heading. http://www.britishcomics.com/images/braun-340-shaver-manual.pdf

Register a free business account Please try your search again later.The power glider recliner mechanism is both smooth, quiet and easy to operate. The baldy is incredibly functional, very comfortable and is perfect for the office, den, or family room.Amazon calculates a product’s star ratings based on a machine learned model instead of a raw data average. The model takes into account factors including the age of a rating, whether the ratings are from verified purchasers, and factors that establish reviewer trustworthiness. A new reference text Epigenetics: A Reference Manual, published by Caister Academic Press and edited by Jeffrey M. Craig and Nicholas C. Wong (Developmental Epigenetics Group, Murdoch Children's Research Institute, Victoria, Australia), presents a current and comprehensive look into the many facets of epigenetics research. The information targets a wide scientific audience and accommodates the expectations of both the novice and expert alike. Scientists with an inquisitive interest in epigenetics will appreciate the thorough description of epigenetic mechanisms with little a priori knowledge required. Scientists working in the field will find the current techniques, description of the technologies and resource tools valuable. With an ever evolving and expanding research field, the established epigenetic investigator will appreciate a centralized tome containing reviews across epigenetic mechanism and model system. Keywords:: epigenetic inheritance, histone modification, telomeres, DNA methylation, epigenetic memory, RNA modification, epigenetic bioinformatics To learn about our use of cookies and how you can manage your cookie settings, please see our Cookie Policy. By closing this message, you are consenting to our use of cookies. Please check your inbox, and if you can’t find it, check your spam folder to make sure it didn't end up there. Please also check your spam folder. http://plenar.hr/wp-content/plugins/formcraft/file-upload/server/content/files/16284016fc2a1e---Buell-firebolt-xb12r-manual.pdf

The Banding Offices acknowledge these changes and uses the common names assigned by the AOU. The AOU stopped using species numbers with the 7th edition of the checklist, but the BBL has continued using the numbers from the 6th edition with modifications. However, we have maintained the common names and species numbers where the AOU has combined formerly acknowledged species. The taxa for which we have included formerly recognized species are: Townsend's Shearwater, Green-winged Teal, Snow Goose, Canada Goose, Brant, Tundra Swan, Great Blue Heron, Red-tailed Hawk, Northern Flicker, Savannah Sparrow, Seaside Sparrow, White-crowned Sparrow, Dark-eyed Junco, Yellow-rumped Warbler, and Palm Warbler. They are not required or recommended for any other purpose. This makes the species list complete for these users. GoIf more than one band size is listed, the preferred recommended band size is listed first. Report suspected parentage as a remark (e.g. Species1 x Species2). Lock-on bands recommended. Strix occidentalis X S. varia. Explain in remarks what the parent species were. All this right in your inbox! At the same time, I accept the Newsletter Subscription Terms. Consent may be revoked at any time. We process the information you provide for the newsletter. Learn more about privacy policies. Your Review Own this LEGO set. Tell everyone what you like about it - add a review. Name: Review: Rating: 1 2 3 4 5 Share this with your readers. HTML code: LEGO 4161 Girl's Freestyle Oval Suitcase, 6 Plus Set Parts Inventory and Instructions - LEGO Reference Guide Ask Toy Tech Latest Story: What Makes LEGO So Popular (LEGO) LEGO, LEGOS, LEGOLAND, TECHNIC, and the LEGO logo are registered trademarks of The LEGO Group, which does not sponsor, authorize, or endorse this site. All images of LEGO products and scans of original building instructions are copyright The LEGO Group. www.dubaimotorcycletours.com/uploaded_images/files/canon-dc19-camcorder-manual.pdf

When you click on links to various merchants on this site and make a purchase, this can result in this site earning a commission. Affiliate programs and affiliations include, but are not limited to, the eBay Partner Network. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally. Bio-Techne WHERE SCIENCE INTERSECTS INNOVATION TM Close Sense probe is reverse complent to the corresponding antisense probe.Not for diagnostic use. Refer to appropriate regulations. RNAscope is a registered trademark; and HybEZ, EZ-Batch and DNAscope are trademarks of Advanced Cell Diagnostics, Inc.Contact Us Downloads OK X Contact Us Complete one of the two forms below and we will get back to you.I further understand that any data provided to Bio-Techne and its brands will be stored, used, and deleted consistent with Bio-Techne’s Privacy Notice Advanced Cell Diagnostics Our new headquarters office starting May 2016: 7707 Gateway Blvd. You have already Quick ordered an Item in your cart. If you want to add a new item, Quick ordered Item will be removed form your cart. Do You want to continue? OK Cancel. Please upgrade your browser to improve your experience. You can choose whether to manage these or allow them all. View Cookie Policy. Something went wrong. I had an identical one for over ten years until the motor wore out. I've had other brands that sound like farm equipment, but this one is the best. There should be a lid but I didn’t notice from the picture that the lid was not included. This should have been disclosed in the written description as the lid is clear plastic and easy to miss. I intended to give this as a gift but now will not be able to do so. So. {-Variable.fc_1_url-

now have two in my kitchen. Cancel Thanks, we'll look into this. All Rights Reserved. User Agreement, Privacy, Cookies and AdChoice Norton Secured - powered by Verisign. The book was originally printed under Edgar Allan Poe 's name.In addition to writing the preface and introduction, Poe made some significant changes to Wyatt's original text.I wrote the Preface and Introduction, and translated from Cuvier, the accounts of the animals, etc.However, this acknowledgement was not incorporated into the second edition.New York City: Cooper Square Press, 1992. p. 106 ISBN 0-8154-1038-7 NY: Harmony Books, 1995.Baltimore: The Johns Hopkins University Press, 1998. p. 277. ISBN 0-8018-5730-9 Baltimore, MD: Johns Hopkins Press, 1998. p.277. Baltimore: The Johns Hopkins University Press, 1998. p. 275-7. ISBN 0-8018-5730-9 NY: Harmony Books, 1995.By using this site, you agree to the Terms of Use and Privacy Policy. This common industrial waste gas can act as the sole energy and carbon source for the bacterium that converts the low value gaseous components into cellular building blocks and industrially relevant products via the action of the reductive acetyl-CoA (Wood-Ljungdahl) pathway. Current research efforts are focused on the enhancement and extension of product formation in this organism via synthetic biology approaches. However, crucial to metabolic modelling and directed pathway engineering is a reliable and comprehensively annotated genome sequence. These variations were confirmed by Sanger sequencing and subsequent analysis suggested that the discrepancies were sequencing errors in the published genome not true single nucleotide polymorphisms. This was corroborated by the observation that over 90 occurred within homopolymer regions of greater than 4 nucleotides in length. http://www.adler-leitishofen.de/wp-content/plugins/formcraft/file-upload/server/content/files/162840182e5b5e---Buell-manual-air-horn.pdf

It was also observed that many genes containing these sequencing errors were annotated in the published closed genome as encoding proteins containing frameshift mutations (18 instances) or were annotated despite the coding frame containing stop codons, which if genuine, would severely hinder the organism’s ability to survive. Furthermore, we have completed a comprehensive manual curation to reduce errors in the annotation that occur through serial use of automated annotation pipelines in related species. As a result, different functions were assigned to gene products or previous functional annotations rejected because of missing evidence in various occasions. Conclusions We present a revised manually curated full genome sequence for Clostridium autoethanogenum DSM10061, which provides reliable information for genome-scale models that rely heavily on the accuracy of annotation, and represents an important step towards the manipulation and metabolic modelling of this industrially relevant acetogen. To date, the focus has been on the use of lignocellulosic biomass feedstocks. One alternative solution is to develop processes based on acetogenic bacteria such as Clostridium autoethanogenum, whereby carbon is directly captured (in the form of carbon monoxide or carbon dioxide) through anaerobic gas fermentation. Thus, gas fermentation allows the production of low carbon fuels and high-value chemicals without competing for food or land. It therefore represents an extremely versatile platform for the sustainable production of commodity chemicals and fuels. As a means of further understanding this organism, and for its effective exploitation for biofuel and biochemical production by means of metabolic engineering, a draft genome sequence of C. autoethanogenum DSM10061 was first elucidated using 454 GS FLX Titanium and Ion Torrent PMG techniques by Bruno-Barcena et al. autoescuelatosal.com/galeria/files/canon-dc100-service-manual.pdf

Improvements in both sequencing technologies and analysis tools have enabled a higher confidence in the generated genome sequence, and as such the coding sequence annotations also become more accurate and refined. Revisiting existing annotations also allows application of new biological knowledge to previously uncharacterised loci, and in the case of manual annotation, allows the opportunity to standardise features such as enzymes names and functional characterisation, for better integration with models. Our detailed inspection of the Brown et al.Were these frame-shifts genuine it would have the effect of severely debilitating the organism’s capacity to survive. To further understand these apparent frame-shifts, we sequenced a stock of C. autoethanogenum DSM10061, purchased directly from the DSMZ culture collection, using Illumina MiSeq technology and mapped these reads onto the Brown et al.Importantly, many of those genes exhibit important cellular functions including the C1 metabolism underlying gas fermentation. In the following sections, we focus on the identified differences, resulting in altered or newly introduced functional annotations and their consequences for the protein network in C. autoethanogenum, and on the underlying reasons for these discrepancies. We also highlight a possible shortcoming of the PacBio RS II sequencing technology, which has implications for future users wishing to employ this technology for gap closing when performing de-novo sequencing. We demonstrate the importance of employing a further sequencing technology following gap closure by PacBio RS II in order to generate a sequence with a high confidence level, and in doing so we have corrected 142 annotation errors in protein coding sequences brought about through apparent frameshift mutation due to under-called homopolymer regions. This represents an important step towards accurate manipulation of the industrially relevant organism, and which may be reliably used as a basis for the generation of metabolic and genomic models. Results Analysis of the C. autoethanogenum genome by Illumina sequencing reveals 243 discrepancies from the Brown et al.This generated over 3.5 million mapped short reads with an average length of 249.91 base pairs, resulting in an average coverage of 200.96 with a standard deviation of 25.67. To analyse the range of the coverage across the genome, we assessed coverage of specifically the coding regions present in the Brown et al.The results confirmed that 99.85 of CDS’s had coverage of at least 40 reads for 100 of the sequence, and 100 of CDS’s had coverage of at least 40 reads for at least 60 of the sequence. The whole-genome sequencing project for C. autoethanogenum JA1-1 has been deposited at National Center for Biotechnology Information (NCBI) under the accession number CP012395. We were able to map reads using the Brown et al.Interestingly, all but one of these discrepancies were identified as single base pair insertions, and these occurred with a seemingly random distribution across the genome, both in coding and non-coding regions (Fig. 2 ). Large INDELs were also screened for using the CLC genomics workbench, but none were detected. Fig. 1 Distribution of coverage of coding sequences across the genome. A visual representation of the depth of coverage of all coding sequences as generated by the Brown et al.Highlighted areas display the location of an insertion site as detected by our Illumina resequencing of the DSM10061 strain when compared to the Brown et al.The length of the homopolymer where each discrepancy was determined and data collated. The vast majority of discrepancies were found to occur when homopolymer length was between 4 and 8 Full size image Sanger sequencing from both forward and reverse reactions from all samples confirmed our Illumina MiSeq data (Additional file 2 ), indicating that the Illumina sequencing had made the correct calls for these single base discrepancies in our strain versus the Brown et al.Comparison of our sequence to that of published sequences revealed that a high percentage of our discrepancies agreed with the finished genome sequence of C. ljungdahlii and of genes located in the Bruno-Barcena draft genome sequence of C. autoethanogenum We found that of the 225 regions that are present in C. ljungdahlii all instances confirm our C. autoethanogenum finished genome sequence. Furthermore, we performed protein BLAST searches against the amino acid sequences of each discrepancy that occurred within a protein-coding region (142 in all). Of these, 127 coding regions are also present in C. ljungdahlii, and 59 are represented within the contigs of the Bruno-Barcena draft genome sequence. 125 coding sequences from C. ljungdahlii are identical to those found in our finished genome sequence, and the two that were not also did not agree with the Brown et al.Of the 59 coding-regions present within contigs of the Bruno-Barcena draft genome sequence, 55 agreed with our sequencing and the four that did not also did not agree with the Brown et al.In summary, through direct comparison with both the C. ljungdahlii finished genome sequence and the Bruno-Barcena draft genome sequence, we can be confident that our Illumina sequence has called the correct bases in these instances. A detailed review of the automated annotation of these proteins in the Brown et al.Moreover, the majority of these discrepancies (207 out of 243) occurred in homopolymer regions greater than five bases in length (Fig. 3 ) and the change present in each of these occurrences was the insertion of an additional monomer in our Illumina sequence, suggesting a tendency for calling strings of homopolymers short by PacBio technology at the time of publication of the Brown et al.Investigation of the origin reveals a previously undiscovered additional 181 base pair insertion One identified discrepancy occurred at the beginning of the genome sequence assembly, where we observed a 1 base pair (bp) deletion. The start point for the assembly of C. ljungdahlii is in a different location to that of C. autoethanogenum finished genome sequences. The additional bases were present in C. ljungdahlii upstream of the mopI gene, which is in the same location as C. autoethanogenum relative to their CDS. This automatic annotation resulted in 3747 perfect matches, 73 matches with a different length. One example of how our manual annotation differed from that of the automated pipeline used by Brown et al. Here the automated pipeline from the Brown et al. Upon further inspection in Pfam, one large ABC-2 family transporter protein domain was found (E-value 6.8e-31). Similar searches of UniProt and KEGG databases agreed with Pfam, therefore we annotated this gene product as an ABC-2 family transporter. The correction of the previously short-called homopolymer reads through our sequencing efforts gave a fully annotated finished sequence of C. autoethanogenum without the erroneous frame-shift containing annotations which had occurred previously. We have also encountered 131 instances where the annotation of a gene product in the Brown et al. The most common cause for the latter was previous identification of a gene product based on a minor region of coverage with homology to a distantly related species. Through comparison of the results of each protein sequence alignment to a broad selection of databases, we were able in some cases to correct and improve upon the automated annotation, highlighting the importance of manual curation where possible. This has supplied us with a robust and comprehensively annotated sequence for subsequent work with this industrially relevant acetogen (Table 2 ). The complete list of gene products with an altered function from those previously described in the Brown et al.Identification of coding sequences not previously detected in the Brown et al. As a consequence of the correction of multiple frameshift mutations, many coding sequences previously annotated as two separate genes due to an erroneous stop codon have now been rectified into a single coding region, and as such our annotated genome now contains 3969 coding sequences, whereas the Brown et al.Discussion The current greatest technical challenge for creating single closed whole genome sequences is the presence of long stretches of repetitive DNA within those sequences, which hinders the assembly of shorter DNA reads into larger scaffolds and finished whole genome sequences. Thus, the utilisation of PacBio systems for the generation of closed WGS’s from organisms that do not currently have such a sequence is highly advantageous in terms of both time and cost. However, it may still be the case that the PacBio system should ideally be used in conjunction with other forms of sequencing following PacBio assembly, such as Illumina MiSeq and Sanger sequencing, to ensure accuracy of the data, certainly for assemblies performed with earlier iterations of the PacBio technology, as is the case with the dataset in question here. Conclusions The whole genome sequence of C. autoethanogenum presented here-in represents a correction of the sequencing errors present in the previously published closed genome sequence generated primarily from an early iteration of PacBio sequencing technology. It was annotated via an automated pipeline and further curated manually to ensure the quality of annotation. Genomic DNA was quantified with a NanoDrop ND-1000 spectrophotometer (Labtech International) and the quality was determined via agarose gel electrophoresis. Whole genome sequencing was performed using an Illumina MiSeq instrument in the DeepSeq facility at the University of Nottingham. Sequencing data was mapped against the published C. autoethanogenum sequence available in the NCBI database (GenBank: CP006763) using the program CLC Genomics Workbench (CLC Bio; Qiagen). Genome sequencing data generation Genome sequencing was achieved at the DeepSeq next generation sequencing facility at the University of Nottingham. Samples were sequenced using an Illumina MiSeq desktop sequencer, a paired-end approach was taken with reads lengths of 250 base pairs. Sequencing data trimming, filtering and assembly Illumina mate-paired reads were trimmed of their adaptor sequences and filtered for quality using the program CLC Genomics Workbench (v. 7.0.4, CLC bio, Denmark), and subsequently assembled using DSM10061 as a reference sequence (Additional file 4 ). PCR and Sanger sequencing Sanger sequencing trace data is available upon request. All other data sets supporting the results of this article are included within the article (and its additional files). Paris: International Energy Agency (IEA) and Organisation for Economic Co-Operation and Development; 2008. Rome: Food and Agriculture Organization of the United Nations; 2008. Comput Struct Biotechnol J. 2012;3:e201210007. PubMed Production of biofuels from synthesis gas using microbial catalysts.Clostridium ljungdahlii represents a microbial production platform based on syngas.Fermentative production of ethanol from carbon monoxide.Influence of process parameters on growth of Clostridium ljungdahlii and Clostridium autoethanogenum on synthesis gas.